Pilot-scale process to produce bio-pigment from Monascus purpureus using broken rice as substrate for solid-state fermentation

Vishu Chaudhary, Priya Katyal, Anil Kumar Puniya, Harsh Panwar, Maninder Arora, Jaspreet Kaur, Namita Rokana, Nitin Wakchaure, Antonio Raposo, Dele Raheem, Anuj Kumar Poonia

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)


Nowadays, natural bio-pigments have immense importance in food industries owing to their non-toxicity, health benefits and safety. In the present study, natural red bio-pigment was produced at a pilot-scale by optimizing fermenter type and bio-pigment extraction parameters (i.e. concentration, time, and temperature). The tray-type fermenter was out performed for bio-pigment production from Monascus purpureus. The high yield of bio-pigment was extracted with 60% ethanol at 60 °C for 80 min having color value of 49.65 optical density unit (ODU)/g DMS with 30.60% DMS recovery rate. The chromatographical analysis of extract revealed three color forms of bio-pigment i.e. red, orange, and yellow. Physicochemical nutrients analysis of fermented biomass revealed a decrease in carbohydrate content, while an increase in other nutrients were observed. The bio-pigment was sensitive to UV, sunlight and pH > 10. However, extracted red bio-pigment can withstand up to 100 °C temperature and 0.6% (w/v) of various food preservatives. The present study provides low-cost, scale-up production of natural red bio-pigment using agricultural by-product thus serve an alternative to synthetic food colors. The study signifies pilot scale bio-pigment production technology that reduces pigment production cost, additionally providing nutritional good fermented spent biomass.
Original languageEnglish
Pages (from-to)1845-1855
Number of pages11
JournalEuropean Food Research and Technology
Issue number7
Publication statusPublished - Jul 2023
MoEC publication typeA1 Journal article-refereed


  • Broken rice
  • Monascus purpureus
  • Pilot-scale fermentation
  • Red bio-pigment
  • Solid-state fermentation

Field of science

  • Food science


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